A statement from the Donetsk separatists said "we cannot accept the values of the Kiev junta, we have our heroic past going back to World War Two, we are the Russian bear which is waking up".
It added: "Don\’t worry, everything will stay peaceful and orderly. The only problem is if the Kiev junta want war."
At talks in Geneva, Russia, Ukraine, the EU and US agreed
that illegal military groups in Ukraine must leave official buildings, and that there should be an amnesty for anti-government protesters.
But protest leaders occupying government buildings in eastern cities such as Donetsk said the interim authorities in Kiev must step down first, as they had taken power in what they said was an illegal coup.
Ukraine\’s interim authorities have appealed for national unity and promised to meet some of the demands of pro-Russian protesters in the east of the country.
These include the decentralisation of power and guarantees for the status of the Russian language.
In a joint televised address to the nation with interim Prime Minister Arseniy Yatsenyuk, acting President Olexander Turchynov said the Russian language could be granted official status alongside Ukrainian in regions where it was spoken by a majority of the population.
He said the decision had been taken to help reach "peace and accord among the citizens of Ukraine".
The two men also said they would support wider constitutional change, including decentralising more power to local government, and urged people to refrain from violence.
The United States says Russia has a "responsibility" to call on pro-Russian militants occupying government buildings in eastern Ukraine to evacuate.
State Department spokeswoman Jen Psaki said Friday that if Russia does not take steps to de-escalate the situation and implement Thursday\’s deal with Ukraine, aimed at lowering tensions, there will be "consequences."
Psaki said the U.S. rejects those comments and the separatists\’ claim that the new Ukrainian government took power in a coup.
U.S. President Barack Obama said Washington will continue preparing sanctions against Russia in case it does not take steps to de-escalate the situation.
"We are coordinating now with our European allies," the president said. "My hope is that we actually do see follow-through over the next several days but I don\’t think given past performance that we can count on that. And we have to be prepared to potentially respond to what continue to be efforts of interference by the Russians."
The West is backing threats of sanctions with a show of military force. Canada is sending six fighter planes to the region to help bolster NATO defenses.
NATO has ruled out military intervention, but says the deployment is aimed at reassuring allies in eastern Europe.
President Vladimir Putin has said he has "a right" to send troops into Ukraine but hopes he will "not have to exercise that right".
Speaking at an annual televised question-and-answer session in Moscow, Putin added that the use of force by the interim government in Ukraine against pro-Russian separatists was a "grave crime."
Putin said he hoped the crisis would be resolved through dialogue.
He also admitted for the first time that Russian forces had been active in Crimea, which was annexed by Moscow last month. Previously he insisted that the camouflaged, masked gunmen who took over Crimea were a local "self-defence" force.